Poly-L-Lactic in regenerative medicine

FAREZ N, .; DIAZ M, ; ORELLANA MF, ; GARCÍA MORENO MF,; VENEGAS ROJAS FM, ; ZABALZA F,; BENEVIDES DE OLIVEIRA G, ; JARDIN MUNHOZ ; VITELLO XAVIER M,; CHULLO LLERENA V, ; LANDGRAF T, ; MISSANA L,; FELDMAN SARA

Capital Federal

Congreso; Congreso de la Asociación de Osteología y Metabolismo MIneral; 2019

Asociación Argentina de Osteología y Metabolismo Mineral

Biodegradable synthetic polymers represent an important group within the materials used for regenerative biomedical application. We previously synthesized a poly-L-Lactic scaffold using ring opening polymerization of the cyclic dimer (PLLA), which did not possess cytotoxic effects in cell cultures and had lower production costs. Our aim was to verify if this scaffold is useful in bone tissue engineering under in vivo conditions (N: New Zealand female rabbits). 15 N (4 months) were randomly divided into Groups A, B and C (control) (n = 5). A and B were subjected to 6 mm diameter osteo-femoral lesion, and A rabbits were implanted with PLLA at the site of the lesion. An adequate clinical condition and gait was shown in A and B from day 5 on. No differences in biochemical values (hemograms and transaminases) were detected after 1; 5 and 90 days. In A rabbits, both conserved cortices were observed, with continuity. Tomography showed higher calcified areas in A than in B. Material compatible with PPLA was detected on day 90, surrounded by a thick layer of neoformed composite bone. Inside the material there were moderate trabeculae with osteoblast-like cellsAlthough preliminary, our data show that PLLA did not cause rejection, and promoted incipient tissue neoregeneration. Since the PLLA has strength, flexibility and malleability properties, it would have potential application for the development of screws and other types of implants in which it is necessary to repair an injury, without undesirable side effects. PLLA is a promising 3D material to be used in regenerative medicine.