PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Production & purification of a fibrinolytic enzyme from Bionectria sp.
Autor/es:
ROVATI, JI; ARNAU, VG; FIGUEROA, LIC; FARIÑA, JI
Lugar:
Tucumán - Argentina
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2009
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0in; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:8.5in 11.0in; margin:1.0in 1.25in 1.0in 1.25in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.Section1 {page:Section1;} --> Currently, several thrombolytic agents such as urokinase, alteplase (t-PA), and streptokinase (SK) are available for clinical use, although they still suffer significant shortcomings, including requirement of large therapeutic dose, short plasma half-life, limited fibrin specificity, reocclusion and bleeding complications. Fibrinolytic enzymes have been purified from plants and animals. Microbial sources were reported from Bacillus, Aspergillus, Fusarium and Streptomyces. In the present study we attempted the isolation of a novel fibrinolytic enzyme from cultures of Bionectria sp. LY 4.1. The microorganism was grown under submerged culture conditions in shake flask scale. Supernatant was fractionally precipitated with cold acetone. For further purification a gel filtration chromatography was performed. Fibrinolytic activity was determined by the fibrin plate method. To quantify the fibrinolytic activity the amount of tyrosine released per min was determined. The effect of pH and incubation temperature was studied. In vitro activity over human blood clots was also analyzed to verify thrombolytic effect. Results confirmed that the purified fibrinolytic enzyme obtained from cultures of Bionectria sp. LY 4.1 shows thrombolytic effect over human blood clots and may play a vital role in saving the humanity from blood clot related diseases with its unique mode of action and less side effects in future.