Biomaterial Characterization for Translational Regenerative Bone Medicine

MORENO GARCIA, MF; FELDMAN S; MISSANA L,

Joao Pessoa- Paraiba

Congreso; 10º Congresso Latino Americano de Órgãos Artificiais e Biomaterias (COLAOB); 2018

COLAOB

Biomaterial Characterization for Translational Regenerative Bone Medicine . Moreno GarcíaMF1, FeldmanS3, EtchegorryD1, VenaR2, MissanaL1.1.OFUNT-PROIMI-Conicet.2.IBR.3. LABOATEM-MedicalCs.UNRosario.Translational Regenerative Bone Medicine suggest follow international standards, to decrease disconnection between clinical and investigational research. Moreover, ISO TS10993-18/19 were selected to determine biological safety and clinical efficacy from biomaterials manufactured and their use as a medical device. OBJECTIVE: To characterize physical-chemical, morphological and topographical properties (PMT) from new bioengineered material, manufactured in PROIMI-UNT according ISO/TS 10993-18 /19, ASTM F2150-02e1, ANMAT, etc. MATERIALS AND METHODS: Group 1: biomaterial by rhPTH 1-34 (Teriparatide, Lilly) (20 μg) and atelocollagen type I ( 3mg / ml, SIGMA ) (3mg). Group 2: atelocollagen type I (3mg). Group 3: rhPTH 1-34 (Teriparatide, Lilly) (20μg). RESULTS: Fourier transform infra red (FT-IR) and RAMAN demonstrated absorption bands in infrared spectrum at Group 1, related to rh-PTH 1-34 ( Amide III 1250 cm-1, Tryptophan 886.1-874.2 cm- 1, Cysteine 644.4 cm -1, deformations CH 2 -CH 3 1449-1460 cm -1 and CH 3 2915-2936-2960 cm -1). Also, rhPTH presence and its first polypeptide physical-chemical characterization. In addition, scanning electron (SEM) and confocal laser electron (CLSM) microscopes revealed thick package fibers (200 to 400 μm) random distribution, pores presence (500 to 200 μm diameter), with interconnections (100 to 300 μm in length). Topographically showed irregular surfaces, tortuous, flat folds, etc. CONCLUSION: From obtained results, it was characterized physical-chemical, morphological and topographically (PMT) the new material for final medical device. In addition, it was demonstrated that collagen was responsible for pores and interconnections and rhPTH of fibrillar deposits.