PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Removal of toxic compounds by a defined actinobacteria consortium
Autor/es:
COLIN VL; SINELI P; BRICEÑO MUÑOZ G; BENIMELI CS; ANTEZANA PE; APARICIO JD; FUENTES MS
Lugar:
Pucón
Reunión:
Workshop; 3º Taller Latinoamericano sobre Rizobacterias Promotoras del Crecimiento Vegetal. 2º Simposio Internacional Biotecnología y Medioambiente; 2016
Resumen:
The continued human development increased the simultaneous presence of multiple contaminants in the environment. The ultimate goal of remediation is to protect human health and the environment. Among the remediation techniques, the bioremediation represents a promising technique, which used together to metabolically versatile microorganisms such as actinobacteria, represents an interesting strategy for environmental clean-up.The aim of this work was evaluate the ability of an actinobacteria consortium to remove phenanthrene (Ph), lindane (-HCH) or Cr(VI), from liquid systems. For it, Streptomyces sp A5, M7, MC1 and Amycolatopsis tucumanensis AB0 previously selected for its ability to tolerate the studied toxic compounds, were used. The strains were simultaneously inoculated as a microbial consortium, in minimal medium added with Ph (0.1 mM), -HCH (2 mgL−1) or Cr(VI) (52 gL−1), respectively. The cultures were incubated 144 h at 30 ºC, taking samples every 48 h. The residual toxic compounds (ECD-GC, HPLC, colorimetric reagent, 1,5-diphenylcarbazide) and the microbial growth (dry weight), was determined. All tests were carried out in triplicate, with the corresponding controls.The microbial growth detected at the end of the assays was 0.14 ± 0.02 gL-1, 0.57 ± 0.06 gL-1, and 1.55 ± 0.11 gL-1 for Cr(VI), Ph and -HCH, respectively. In the presence of Cr(VI), a biomass declined throughout the assay was observed, suggesting a toxic effect of the metal on the consortium growth. The removal percentages detected were 0%, 52.21% and 83.34% for Cr(VI), -HCH and Ph respectively. The abiotic removal detected in Ph and -HCH controls ranged between 13.5-12.4%. Thus, the biological removal detected was 69.8% for Ph and 39.8% for -HCH.Based upon these findings, the selected consortium could represent a promising tool for bioremediation of liquid systems contaminated with organic compounds such as Ph and -HCH.