PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
SEMINARIO INTERNACIONAL NUEVAS TECNOLOGÍAS PARA LA PROTECCIÓN DEL MEDIO AMBIENTE
Autor/es:
CUOZZO S.,
Lugar:
TEMUCO
Reunión:
Conferencia; SEMINARIO INTERNACIONAL NUEVAS TECNOLOGÍAS PARA LA PROTECCIÓN DEL MEDIO AMBIENTE; 2016
Institución organizadora:
UNIVERSIDAD DE LA FRONTERA
Resumen:
Many Actinobacteria are well known as degraders of toxic organic materials or immobilizing of heavy metal; and are used in bioremediation process where the most abundant group is Streptomyces. Streptomyces sp. M7 possesses ability to tolerate and remove organochlorine pesticides, such as lindane (-HCH) or - and - hexachlorocyclohexane isomers (HCH). The Dehalogenases are key enzymes for the degradation of various halogenated compounds. In the case of γ-HCH with six chlorine atoms where dechlorination, is a very significant step in its degradation process. In fact, we have shown that three different enzymes, LinA (dehydrohalogenases), LinB (Haloalkane dehalogenase), and LinC (alcohol dehydrogenase), are involved in the HCH isomers degradation. The proteins sequence analysis of Streptomyces sp. M7, determined that the active sites were conserved namely Apartic acid: 24; 25 and Histidine: 72, compared with the Lin A of Rhodococcus equi, protein responsible for the cleavage of the chemical bond Cl-H, where the sequences presented one similarity among 30-40%. The LinB protein sequence of Streptomyces sp. M7 was compared with LinB of Mycobacterium tuberculosis H37Rv, showed that the active site was conserved, E (Glutamic 129), D (Aspartic100) and H (Hystidine 290) with one similarity of among 22-38%.However, the function and features of the enzymes are still under investigation. LinC is related the short-chain alcohol dehydrogenase family, where the hypothetical LinC enzyme of Streptomyces sp. M7, conserved the active site: Ser-Tyr-Lys, with one protein similarity of among 39-56%.The Streptomyces strains are able to the bio-remediate of heavy metal through different mechanisms: by the conversion of inorganic compounds into non-toxic products, such as resistant to Cu(II), Cd(II); or also by the mechanisms of Cu(II) and Cd(II) tolerance include biosorption processes and subsequent bioaccumulation.