PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Isolation and Molecular Characterizations of Marine Bacteria isolated from Beagle Channel
Autor/es:
HÉCTOR ANTONIO CRISTÓBAL; MARIA ALEJANDRA LOPÉZ; CARLOS MAURICIO ABATE
Lugar:
Mar del Plata, Argentina
Reunión:
Congreso; XLIII Reunion Anual de la Sociedad Argentina de Investigación en Bioquimica y Biologia Molecular; 2007
Institución organizadora:
Sociedad Argentina de Investigacion en Bioquimica y Biologia Molecular
Resumen:
Marine microorganisms play an important ecological role in the biological process of transformation of marine environment materials. Marine bacteria exhibit different hydrolytic enzymes that degrade fractions of organic matter in the sea. Marine microorganisms are the extremophiles carry out main a role in the biodegradation of the organic matter in diverse Antarctic ecosystems. Those culturing efforts have yielded new species within the divisions of ¥ã- and ¥á-Proteobacteria. The aims of this work were the isolation and taxonomy characterization of marine microorganisms from Antarctic region. Samples of benthonic organisms and seawater were taken from different coastal areas of the Beagle Channel (55¨¬S; 67¨¬W), Tierra del Fuego, Argentina. Samples were spread in LB medium and growth at 4 and 15 ¨¬C. The 95 isolated colonies were characterized by 16S rDNA and gyrB PCR amplifications. Isolates were separated into groups according to their ARDRA profiles and RFLP analysis. 16S rDNA and gyrB gene were sequenced from one or two isolate from each group, and the phylogenetics trees were constructed. The gene sequences allowed us to determine their association with the class Proteobacteria; members of genera Pseudomonas, Pseudoalteromonas, Serratia, Halomonas, Alteromonas, Psychrobacter, Shewanella. These studies provide a new source of strains, genes, enzymes and metabolites for biotechnological applications.