Polymorphism analysis of esterases patterns from Bacillus strains

LOTO, FLAVIA; BAIGORI, MARIO DOMINGO; PERA, LICIA MARÍA

Tafi del Valle, Tucumán, Argentina

Jornada; XXIII Annual Scientific Meeting; 2007

Tucumán Biology Society

Introduction: Electrophoretic analysis of esterases (EC 3.1.1.1) patterns is one of the easiest ways to evaluate enzyme variants, providing valuable information in the study of species differentiation. Objective: The aim of this work was to analyze the electrophoretic profiles of esterases from Bacillus strains. Materials and methods: Microorganisms isolated from different sources were grown on LB agar during 48h. Extracellular esterases were extracted from the medium and chromatographied under nondenaturing conditions. Activities were detected using á-naphtyl acetate as substrate. Esterases were classified according to the band migration rate. They were recorded based on presence/absence in the nPAGE. The degree of similarity was estimated by simple matching coefficients. Clusters were then constructed using the UPGMA method. Results and conclusions: Fifteen different bands from fifty isolations were detected. A numerical classification reveals three main clusters with eighteen different enzymatic profiles. There was no correlation between the enzymatic profile and the source of strain isolation. This work was supported by grants PIP 6062 and PICTO 761.