PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Bone Formation from rhPTH and Collagen Matrix in a 3-Dimensional Mesh Scaffold.
Autor/es:
MARÍA V. JAMMAL ; LILIANA R. MISSANA.
Lugar:
Rosario de Santa Fe
Reunión:
Congreso; 8vo Congreso Latinoamericano de Organos Artificiales, Biomateriales e Ingeniería de Tejidos (COLAOB); 2014
Institución organizadora:
Sociedad Latinoamericana de Organos Artificiales, Biomateriales e Ingeniería de Tejidos
Resumen:
Recombinant Human Parathyroid Hormone (rhPTH 1-34) is widely applied for osteoporosis treatment. The aim of this study was to evaluate bone regeneration ability of rhPTH and atelocollagen type I membrane applied in calvarial critical size defect (CZD). 44 Wistar female rats (body weight 150 ± 50 g) with CZD (5mm) were divided into 4 groups: group 1: rhPTH (rhPTH); group 2: atelocollagen (C); group 3: rhPTH and atelocollagen I (CrhPTH); group 4: without treatment (CG). All samples were evaluated at 1st, 3rd and 6th week (wk) post-surgery by soft X-ray, histological and histometric studies. Soft X-ray results showed many irregular radiopaque areas. Histologically, rhPTH was replaced by reticular bone (7%) at 3rdwk, and with lamellar bone ossicles (30%) at 6th wk. C showed bone formation similar to composite bone on 1st, 3rd and 6th wk (2%, 44% and 41% respectively). With CrhPTH, bone formation was observed in all periods (2.4%, 48% and 53%), showing statistical difference with CG in the 3rd and 6th wk (p= 0.03 and 0.01). The highest percentage of bone obtained with CrhPTH would be explained by a synergistic effect between both proteins. Our study provides valuable information from a tissue engineering tool and its application for bone defect regeneration.