PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
RESISTANCE PROFILES AND BIOFILM FORMATION BY EXTREMOPHILES ISOLATED FROM HIGH-ALTITUDE ANDEAN LAKES
Autor/es:
FEDERICO ZANNIER; OMAR ORDOÑEZ; LUCIANO R. PORTERO; FARIAS MARIA EUGENIA; ALBARRACIN VIRGINIA H.
Lugar:
San Miguel de Tucumán
Reunión:
Jornada; XXXI Jornadas Científicas de la Asociación de Biología de Tucumán; 2014
Resumen:
High-Altitude Andean Lakes (HAAL) at northwest Argentina are ecosystems located between 3,000 and 6,000 m and exposed to a wide variety of extreme conditions: i.e. high UV radiation (UV), hipersalinity, daily wide range of temperature, and high concentration of toxic elements such as arsenic (As). Nevertheless, extremophilic microbial communities adapted at such conditions thrive there, which have great biotechnological potential mainly as source for novel antibiotics and alternatives for bioremediation.      The aim of this work is to assess resistance profiles of Acinetobacter sp. Ver3, Exiguobacterium sp. S17 and Exiguobacterium sp. N39 towards arsenic and UV-B exposure. The biofilm formation in different culture conditions is also investigated. The resistant profile to UV was measured in LB agar media under increasing doses of UV-B while the tolerance to As was studied in LB liquid media at different concentrations of arsenite (As III) and arsenate (As V). The production of biofilm was tested on different supports and culture media and quantified. The results were compared with strains from the DSMZ German Culture Collection; A. johnsonii DSM6963 and E. arantiacum DSM6208. All strains were selected from LIMLA-PROIMI-CONICET Microbial Collection. Our results showed that HAAL bacteria are much more resistant than the control strains to both UV-B and arsenic. In the other hand, biofilm production was more significantly when the strains were cultured in LB media supplemented with glycerol. The present study demonstrated the ability of HAAL strains to endure physico-chemical stress and to develop a resistant phase (biofilm) in different experimental conditions. These findings represent the experimental basis for the potential development of biofilm from extremophiles cultures for application in bioremediation process.