PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
RESISTANCE PROFILES AND BIOFILM FORMATION BY EXTREMOPHILES ISOLATED FROM HIGH-ALTITUDE ANDEAN LAKES
Autor/es:
FEDERICO ZANNIER; OMAR ORDOÑEZ; LUCIANO R. PORTERO; FARIAS MARIA EUGENIA; ALBARRACIN VIRGINIA H.
Lugar:
San Miguel de Tucumán
Reunión:
Jornada; XXXI Jornadas Científicas de la Asociación de Biología de Tucumán; 2014
Resumen:
High-Altitude
Andean Lakes (HAAL) at northwest Argentina are ecosystems located between 3,000
and 6,000 m and exposed to a wide variety of extreme
conditions: i.e. high UV radiation (UV), hipersalinity, daily wide range of
temperature, and high concentration of toxic elements such as arsenic (As). Nevertheless,
extremophilic microbial communities adapted at such conditions thrive there,
which have great biotechnological potential mainly as source for novel
antibiotics and alternatives for bioremediation.
The aim of
this work is to assess resistance profiles of Acinetobacter sp. Ver3, Exiguobacterium
sp. S17 and Exiguobacterium sp. N39
towards arsenic and UV-B exposure. The biofilm formation in different culture
conditions is also investigated.
The resistant
profile to UV was measured in LB agar media under increasing doses of UV-B
while the tolerance to As was studied in LB liquid media at different
concentrations of arsenite (As III) and arsenate (As V). The production of
biofilm was tested on different supports and culture media and quantified. The
results were compared with strains from the DSMZ German Culture Collection; A. johnsonii DSM6963 and E. arantiacum DSM6208. All strains were
selected from LIMLA-PROIMI-CONICET Microbial Collection.
Our results
showed that HAAL bacteria are much more resistant than the control strains to both
UV-B and arsenic. In the other hand, biofilm production was more significantly
when the strains were cultured in LB media supplemented with glycerol.
The present
study demonstrated the ability of HAAL strains to endure physico-chemical
stress and to develop a resistant phase (biofilm) in different experimental
conditions. These findings represent the experimental basis for the potential
development of biofilm from extremophiles cultures for application in
bioremediation process.