PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Evaluation of gyrb sequences for taxonomic analices of heavy metal resistant actinomycetes
Autor/es:
ALBARRACÍN VH,; POLTI MA; AMOROSO MJ; ABATE CM
Lugar:
Rosario, Santa Fé
Reunión:
Congreso; XLII Reunion Anual-SAIB; 2006
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
A copper resistant actinomycete strain AB0, previously isolated from polluted sediments, has the ability of reducing copper present in a synthetic medium in a 70 %  after seven days of growth. Advances in understanding the way in which this microorganism accumulates the metal as well as its characterization are very important to develop novel copper bioremediation processes. The aim of this work is to demonstrate the copper accumulation capacity of this strain using a transmission electron microscopy histochemical technique as well as performing a fully completed characterization of this strain. Using the Timm´s reagent, Cu+ appeared as reaction deposits of reduced silver within the cell, mostly in the tips where typical actinomycete apical growth is produced but some metal deposits were also found associated with the exopolimer. Morphological and biochemical assays were performed using microtiter techniques thats includes lisozime and NaCl resistance, melanin production, pH tolerance, carbohydrate assimilation. According to the obtained results and 16s rDNA sequencing, the isolated strain was characterized as Amycolatopsis sp. AB0.