PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Study of parasporal inclusions by numerical analysis of their SDS-PAGE proteins
Autor/es:
ALVAREZ ANALIA; PERA, LICIA M; VIRLA EDUARDO; BAIGORI M.D
Lugar:
Rosario. Santa Fe, Argentina
Reunión:
Congreso; SAIB 42 th Annual Meeting. XLII Reunión Anual; 2006
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Bacillus thuringiensis(Bt) is a Gram-positive, endospore-forming bacterium that produces proteinaceous parasporal inclusions during sporulation. These inclusions often contain ä-endotoxin proteins that cause rapid and fatal intoxication of several insect orders. It has been established that the inclusions proteins, encoded by cry genes, are highly heterogeneous in structures and biological activities. We previously reported a native Bt RT3 that shows a strong toxicity against Spodoptera frugiperda(Bt) is a Gram-positive, endospore-forming bacterium that produces proteinaceous parasporal inclusions during sporulation. These inclusions often contain ä-endotoxin proteins that cause rapid and fatal intoxication of several insect orders. It has been established that the inclusions proteins, encoded by cry genes, are highly heterogeneous in structures and biological activities. We previously reported a native Bt RT3 that shows a strong toxicity against Spodoptera frugiperdaä-endotoxin proteins that cause rapid and fatal intoxication of several insect orders. It has been established that the inclusions proteins, encoded by cry genes, are highly heterogeneous in structures and biological activities. We previously reported a native Bt RT3 that shows a strong toxicity against Spodoptera frugiperdaSpodoptera frugiperda larvae. The aim of this work was to study parasporal inclusions by numerical analysis of their SDS-PAGE proteins profiles. Standard B. subtilis (1A571) and Bt strains (BGSC): 4A4, 4D3 and 4D1 as well as native strains were used throughout this study. Proteins were prepared by a rapid washing procedure. SDS-PAGE of standards and inclusions proteins was performed according to Laemmli method. Gels were stained with silver reagent and they were analyzed by using NTSYS program (SM coefficient and UPGMA). Two main groups of Bacillus strains can be differentiated showing a similarity of 90 % between them. Protein profiles from native strains were different from those of standard strains. Bt RT3 displays a similarity of 92% with Bt 4D1. This method was more sensitive to differentiate Bt strains than a set of biochemical tests. This work was supported by grants PICTO 761 and PIP 6062.B. subtilis (1A571) and Bt strains (BGSC): 4A4, 4D3 and 4D1 as well as native strains were used throughout this study. Proteins were prepared by a rapid washing procedure. SDS-PAGE of standards and inclusions proteins was performed according to Laemmli method. Gels were stained with silver reagent and they were analyzed by using NTSYS program (SM coefficient and UPGMA). Two main groups of Bacillus strains can be differentiated showing a similarity of 90 % between them. Protein profiles from native strains were different from those of standard strains. Bt RT3 displays a similarity of 92% with Bt 4D1. This method was more sensitive to differentiate Bt strains than a set of biochemical tests. This work was supported by grants PICTO 761 and PIP 6062.Bacillus strains can be differentiated showing a similarity of 90 % between them. Protein profiles from native strains were different from those of standard strains. Bt RT3 displays a similarity of 92% with Bt 4D1. This method was more sensitive to differentiate Bt strains than a set of biochemical tests. This work was supported by grants PICTO 761 and PIP 6062.