PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Study of parasporal inclusions by numerical analysis of their SDS-PAGE proteins
Autor/es:
ALVAREZ ANALIA; PERA, LICIA M; VIRLA EDUARDO; BAIGORI M.D
Lugar:
Rosario. Santa Fe, Argentina
Reunión:
Congreso; SAIB 42 th Annual Meeting. XLII Reunión Anual; 2006
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Bacillus thuringiensis(Bt) is a Gram-positive, endospore-forming
bacterium that produces proteinaceous parasporal inclusions
during sporulation. These inclusions often contain ä-endotoxin
proteins that cause rapid and fatal intoxication of several insect
orders. It has been established that the inclusions proteins,
encoded by cry genes, are highly heterogeneous in structures
and biological activities. We previously reported a native Bt RT3
that shows a strong toxicity against Spodoptera frugiperda(Bt) is a Gram-positive, endospore-forming
bacterium that produces proteinaceous parasporal inclusions
during sporulation. These inclusions often contain ä-endotoxin
proteins that cause rapid and fatal intoxication of several insect
orders. It has been established that the inclusions proteins,
encoded by cry genes, are highly heterogeneous in structures
and biological activities. We previously reported a native Bt RT3
that shows a strong toxicity against Spodoptera frugiperdaä-endotoxin
proteins that cause rapid and fatal intoxication of several insect
orders. It has been established that the inclusions proteins,
encoded by cry genes, are highly heterogeneous in structures
and biological activities. We previously reported a native Bt RT3
that shows a strong toxicity against Spodoptera frugiperdaSpodoptera frugiperda
larvae. The aim of this work was to study parasporal inclusions
by numerical analysis of their SDS-PAGE proteins profiles.
Standard B. subtilis (1A571) and Bt strains (BGSC): 4A4, 4D3
and 4D1 as well as native strains were used throughout this
study. Proteins were prepared by a rapid washing procedure.
SDS-PAGE of standards and inclusions proteins was performed
according to Laemmli method. Gels were stained with silver
reagent and they were analyzed by using NTSYS program (SM
coefficient and UPGMA). Two main groups of Bacillus strains can
be differentiated showing a similarity of 90 % between them.
Protein profiles from native strains were different from those of
standard strains. Bt RT3 displays a similarity of 92% with Bt 4D1.
This method was more sensitive to differentiate Bt strains than a
set of biochemical tests. This work was supported by grants
PICTO 761 and PIP 6062.B. subtilis (1A571) and Bt strains (BGSC): 4A4, 4D3
and 4D1 as well as native strains were used throughout this
study. Proteins were prepared by a rapid washing procedure.
SDS-PAGE of standards and inclusions proteins was performed
according to Laemmli method. Gels were stained with silver
reagent and they were analyzed by using NTSYS program (SM
coefficient and UPGMA). Two main groups of Bacillus strains can
be differentiated showing a similarity of 90 % between them.
Protein profiles from native strains were different from those of
standard strains. Bt RT3 displays a similarity of 92% with Bt 4D1.
This method was more sensitive to differentiate Bt strains than a
set of biochemical tests. This work was supported by grants
PICTO 761 and PIP 6062.Bacillus strains can
be differentiated showing a similarity of 90 % between them.
Protein profiles from native strains were different from those of
standard strains. Bt RT3 displays a similarity of 92% with Bt 4D1.
This method was more sensitive to differentiate Bt strains than a
set of biochemical tests. This work was supported by grants
PICTO 761 and PIP 6062.