PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The linear plasmids of Micrococcus luteus and the resistance to extreme conditions
Autor/es:
DIB, JULIÁN RAFAEL; WAGENKNECHT, MARTIN; THÜRNER, ANDREA; DANIEL, ROLF; FARÍAS MARIA EUGENIA; MEINHARDT, FRIEDHELM
Lugar:
Münster
Reunión:
Conferencia; Actinobacteria within solis; 2012
Institución organizadora:
FEMS
Resumen:
Numerous bacterial species colonize adverse or even hostile habitats. The ability to thrive in extreme environments frequently comes along with the existence of plasmids. Micrococcus luteus strains A1 and V7, recently isolated from high-altitude wetlands in the northwest of Argentina (1), in fact were found to harbor large linear plasmids (2). To check whether such elements may confer the genetic equipment permitting survival under the prevailing extreme conditions, plasmids pLMA1 (110 kb) and pLMV7 (90 kb) were sequenced. Sequencing was either performed solely with the Sanger technology (pLMV7) or in a combination of the former with 454 pyrosequencing (pLMA1) (Roche, Mannheim). Obtained sequences were analyzed by applying standard bioinformatics tools. We determined draft sequences of plasmid pLMA1 and pLMV7 (termini not yet complete). In silico analyses suggest 126 open reading frames (ORFs) for the former and 108 for the latter element, respectively. Conspicuously, both of the extrachromosomal genetic elements include a large number of ORFs encoding putative transposases, some of which resembling polypeptides known from the Micrococcus chromosome. Moreover, ORFs potentially conferring resistance to erythromycin, tetracycline and universal stress family proteins were identified as well as sequences coding for proteins involved in replication, conjugation, and DNA repair. From these results, we can conclude that the preponderance of transposase encoding open reading frames suggests pLMA1 and pLMV7 to be rather adaptable genetic elements. And, in line with the above conclusion is the plasmids? coding potential which agrees with the extreme environmental conditions to which their hosts are exposed. References: 1. Dib J, Motok J, Fernandez Zenoff V, Ordoñez O and Farías M E. (2008) Current Microbiology 56(5):510-7. 2. Dib J R, Wagenknecht M, Hill R, Farías M E and Meinhardt F (2010) Plasmid; 63: 40-45.