PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Peroxiredoxin proteins over-expression by Candida fukuyamaensis RCL-3 under copper overload.
Autor/es:
VERÓNICA IRAZUSTA; LUCAS MICHEL; LUCÍA INÉS FIGUEROA DE CASTELLANOS
Lugar:
Madison, Wisconsin
Reunión:
Congreso; 13th International Congress on Yeasts; 2012
Resumen:
Copper (Cu) plays an essential role in cellular
metabolism due to its versatility as a biological catalyst. It is required as a
catalytic cofactor in many enzymes involved in diverse cellular processes.
While trace amounts of copper are essential for life, copper can easily react
with oxygen or hydrogen peroxide (H2O2) generating
reactive oxygen species (ROS) that may damage cell constituents through the
oxidation of proteins, cleavage of DNA and RNA, and lipid peroxidation. In
order to counteract this adverse aspect, all living organisms have developed
defense mechanisms against ROS, allowing them to live under different stress
situations. Candida fukuyamaensis
RCL-3 (NCBI number AY743221), yeast strain isolated from a copper filter plant
at the province of Tucumán, Argentina, has the ability of supporting high
amounts of copper metal by a slowdown in its growth rate.
In order to understand the mechanism involved in C. fukuyamaensis RCL-3 resistance to copper it was
conducted a proteomic approach. Atomic absorption spectroscopy
results showed decrease copper concentration (from 0.5 to 0.14 ± 0.05 mM) in the culture
medium after 16 h inoculation. Crude cell extracts mono-dimensional gel
electrophoresis revealed differential bands expressions between cells grown
with or without copper. Further, with the aim of studying these differences,
two-dimensional electrophoresis analyses was carried out. Gels analyzed with
Image Master Program. 2-D analysis of C. fukuyamaensis
RCL-3 revealed that 16 h copper exposure produced an over-expression of 40
proteins. Four of this proteins identified by MALDI TOF/TOF were found as peroxiredoxin
family members.
The results obtained in the present work show that when exposing C.
fukuyamaensis RCL-3 to 0.5mM copper concentration, it
is produced a differential protein expressions probably involved in cell
resistance mechanisms.