PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Simultaneous Cu(II) and Cr(VI) Removal by Single and Mixed
Autor/es:
RODRIGUEZ, A; ALVARENGA A; COLIN, V L; VILLEGAS L B; AMOROSO M J; ABATE C M
Lugar:
Puerto Vallarta
Reunión:
Congreso; ISBA XVI. The evolution of Actinomycetes; 2011
Resumen:
Heavy metal pollution is one of the most serious environmental problems because they are not chemical and not biology degraded therefore they can remain for a long time in the nature. Microorganisms play a significant role in bioremediation of heavy metal contaminated soil and wastewater. Actinobacteria are known for their ability to colonize soil and they constitute a significant component of the microbial population in it. Previous studies with actinobacteria reported the potential capacity of them, as tools for Cu(II), Cr(VI) or Cd(II) bioremediation. Normally soil or sediments present problems of cocontamination with various heavy metals. The results obtained leads us to study the possibility to use single and mixed cultures of actinobacteria for Cu(II) and Cr(VI) removal in liquid medium simultaneously. Amycolaptosis tucumanensis, Streptomyces sp. MC1, Streptomyces sp. F4 isolated from contaminated sediments and characterized in before works and two new strains (called GHB and TC1) isolated from Tucumán, which also showed heavy metal tolerant were used. Strains inhibition test were done on different solid medium. TC1 showed inhibition activity in some medium. Therefore single and mixed cultures with and without TC1 were used. Cells were incubated in Minimal Medium with glucose as only carbon source and supplemented with 20 ppm of Cu(II) and Cr(VI). Cultures were incubated during 144 h at 30 ºC and 160 rpm. Cells were harvested each 24 h and biomass was determined by dry weight. Cr(VI) removal activity was measured by the colorimetric method of 1,5-diphenylcarbazide and total chromium and copper by Atomic Absorption in the supernatant. Mixed Cultures without TC1 were more effective in Cr(VI) removal but did not show significant differences on Cu(II) removal with single cultures. These results show that mixed cultures are a better tool than single cultures in Cu(II) and Cr(VI) removal when they are at the same time. Amycolaptosis tucumanensis, Streptomyces sp. MC1, Streptomyces sp. F4 isolated from contaminated sediments and characterized in before works and two new strains (called GHB and TC1) isolated from Tucumán, which also showed heavy metal tolerant were used. Strains inhibition test were done on different solid medium. TC1 showed inhibition activity in some medium. Therefore single and mixed cultures with and without TC1 were used. Cells were incubated in Minimal Medium with glucose as only carbon source and supplemented with 20 ppm of Cu(II) and Cr(VI). Cultures were incubated during 144 h at 30 ºC and 160 rpm. Cells were harvested each 24 h and biomass was determined by dry weight. Cr(VI) removal activity was measured by the colorimetric method of 1,5-diphenylcarbazide and total chromium and copper by Atomic Absorption in the supernatant. Mixed Cultures without TC1 were more effective in Cr(VI) removal but did not show significant differences on Cu(II) removal with single cultures. These results show that mixed cultures are a better tool than single cultures in Cu(II) and Cr(VI) removal when they are at the same time.