PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Catalytic properties of constitutive and olive oil-induced extracelular and mycelium-bound lipases from Penicillium corylophilum
Autor/es:
ROMERO, CINTIA MARIANA; PERA, LICIA MARÍA; BARON, ALESSANDRA; KRIEGER, NADIA; BAIGORI, MARIO DOMINGO
Lugar:
Trivandrum
Reunión:
Congreso; New horizons in biotechnology (NHBT); 2011
Institución organizadora:
New horizons in biotechnology (NHBT)
Resumen:
Aim: To study the biocatalytic properties and stability of extracellular and mycelium-bound lipases produced by Penicillium corylophilum cultured in either presence or absence of olive oil. Methods and Results: Screening of extracellular and mycelium-bound lipase allowed selection of P. corylophilum. Lipase production in a submerged culture containing 2% olive oil increased 2.6 and 1.4 fold extracellular and mycelium-bound lipase, respectively, compared with constitutive conditions. In general, the four biocatalyst systems showed high residual activity at acid and neutral pH. Preincubation at 40°C caused an increase in the extracellular olive oil-induced lipolytic activity. On the contrary, residual activity of extracellular constitutive lipase decreased within the 30–55°C range. Constitutive mycelium-bound lipase showed to be active at high temperatures like 50 and 55°C, while the activity of olive oil-induced mycelium-bound lipase decreased at 55°C. Constitutive and olive oil-induced extracellular lipase showed highest stability in water-miscible solvents. Constitutive and olive oil-induced mycelium-bound lipase showed residual activity in both water-miscible and immiscible solvents. Conclusions: Different catalytic properties of several biocatalysts from the same microorganism were obtained by changing the surrounding medium. Significance and impact of the study: Selectively modified biocatalysts could be used for different industrial process cheapen the costs of the biocatalysis.