PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Plasmids in Archaea from extreme high altitude Andean lakes (HAAL)
Autor/es:
FARÍAS, MARIA EUGENIA; DIB, JULIÁN RAFAEL; MALDONADO, MARCOS JAVIER
Lugar:
Bariloche
Reunión:
Conferencia; International Plasmid Biology Conference; 2010
Resumen:
The aim of this study was to examine plasmids in halophilic archaea in high altitude lakes from the Andes Mountains and Puna in South America. Puna is a unique natural area covering northern Chile, western Bolivia, central and southern Peru and northwestern Argentina (NOA). Halophilic extreme microorganisms live in natural environments or in evaporation ponds of salt water, where salt concentration is very high (up to 5 M or 25 %). Water and sediments samples were collected from Tolar Grande and Socompa (Salta, Argentina) to perform physicochemical analysis and culture enrichments; these lakes are located between 3,000 and 4,500 m. Sediments and water samples were plated in 6WS and GN101 media. Plasmid DNA was isolated by using a modified method of Anderson and McKay.  Seven of the isolates contain plasmids, AT4 (Natrialba sp.), AT5 (Natrialba sp.), AT6 (Natrialba sp.), AT8 (Natrialba sp.), AT9 (Natrialba sp.), AC10 (Natrialba sp.), AC12 (Natrialba sp.), and AT23 (Halopiger sp.) with sizes ranging from 1.5 kb to 9 kb approximately. Apparently the presence of plasmids in Archaea from high altitude lakes is a widespread phenomenon and DNA sequencing has been performed in order to reveal resistance genes to these extreme environments or other biotechnological features.