INVESTIGADORES
BONGIOVANNI Guillermina Azucena
congresos y reuniones científicas
Título:
Multiememental mapping and arsenic determination in organs of rats by SR-µXRF
Autor/es:
BONGIOVANNI, GUILLERMINA; PEREZ, CA; RUBATTO BIRRI, PN; RUBIO, M; PEREZ, RD
Lugar:
Campinas, Sao Paulo, Brasil
Reunión:
Otro; 16 Reunión Anual de Usuarios de Síncrotron; 2006
Institución organizadora:
Laboratorio Nacional de Luz Síncrotron
Resumen:
Synchrotron microscopic x-ray fluorescence analysis (m-SRXRF) was applied for determining the two-dimensional distribution of Cl, K, Fe, Cu, Zn, As and Br concentrations in kidney rat sections. The animals received drinking water containing 100ppm of sodium arsenite ad libitum for 30 and 60 days.  Lyophilized kidney sections prepared from normal and treated rats were scanned with a collimated white synchrotron spectrum (300 mm ´ 300 mm). The accumulation of arsenic and copper in rat kidney induced by arsenic exposure was corroborated and the spatial distributions of these elements were studied in detail. While copper was restricted to renal cortex, arsenic shown changes in its spatial distribution suggesting nephrotoxicity. A correlation between zinc and arsenic spatial distributions was observed which seems to be caused by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions under arsenic exposure showing a correlation probably to maintain electrical neutrality. There were not significant changes in iron and bromine but the patterns of their spatial distributions were clearly identified. The obtained results shown that m-SRXRF may be a useful tool to detect the spatial distributions of elements in mammal tissues on health and diseased conditions, which suggest interesting hypothesis.