INVESTIGADORES
PEREZ Ethel Erminia
congresos y reuniones científicas
Título:
Taguchi?s Methodology for determining optimum operating conditions in aqueous enzymatic extractions applied to sunflower ?collets
Autor/es:
RODRIGUEZ L.M.; PEREZ E. E.; FERNÁNDEZ M. B. ; CRAPISTE G. H.
Lugar:
Rosario
Reunión:
Congreso; World Congress on oil & fats 31st ISF Lectureship Series (XVI Congreso Latinoamericano de Grasas y Aceites); 2015
Institución organizadora:
AOCS - ASAGA
Resumen:
Industrial processes for the recovery oil from sunflower seeds are usually achieved by conventional mechanical pressing, thus obtaining a meal cake or expeller with a15% of oil, followed by extraction with organic solvents. Aqueous enzymatic extraction has become a clean and less energy consuming technological process to obtain oil from different feedstocks. The enzymes are able to breakdown cell structure of plants, making it permeable, and to release the oil from cells. The aim of the present work was to determine the optimum operating conditions in aqueous enzymatic extractions applied to sunflower ?collets?, measured as the ability to release oil (% db) from the solid , reducing sugar content (mg glucose/mL) in the resulting supernatant extraction. Samples were characterized by humidity, oil content, protein, and structural fibers. Treatment was performed in a stirred batch system, controlled at 50 °C, using enzyme VISCOZYME L. The experiments were carried out using different buffers (acetate, phosphate and citrate), exposition times (1, 2 and 3 hours) and relationship enzyme:sustrate (E/S = 1/5, 1/10, and 1/20). The Taguchi method was followed in order to explore the optimum operating conditions by using an L9 experimental design, and select the most favorable levels of each variable, considered as the best alternative, the one that minimizes the content of residual oil in the meal. The selection of optimum experiment, using a relationship E/S of 1/20 and a time of 1 hour, generated an oil content in the residual meal of 27.48 % (db); under these conditions the reducing sugar content in the supernatant was 10.1 mg Glucose/mL of supernatant. The type of buffer showed no significant differences.