Dynamic Flux Balance Analysis in Cyanobacteria for Ethanol Production with Simultaneous Optimization Approaches

DELPINO, C.; ESTRADA, V.; LAIGLECIA, J.; VIDAL VIDAL, R.; FLORENCIO BELLIDO, F. J.; GARCÍA GUERRERO, M.; DÍAZ, M. S.

Computer Aided Chemical Engineering

ELSEVIER

Lugar: Amsterdam; Año: 2014 vol. 33 p. 1165 - 1170

1570-7946

In this work we address dynamic optimization of ethanol production by Dynamic Flux Balance Analysis in an engineered cyanobacterium with autotrophic growth (i.e., using CO2 as substrate). The photobioreactor optimization model is integrated to the metabolic network one by replacing the inner problem by its first order optimality conditions. Complementarity constraints that arise associated to the optimality conditions are efficiently handled with a penalty function formulation. Numerical results suggest the possibility to activate the ethanol production pathway after 20 h in the batch run to enhance ethanol production.