INVESTIGADORES
FERREIRA Maria Lujan
congresos y reuniones científicas
Título:
Influence of His-tag on activity, stability and immobilization of the lipase LipC12
Autor/es:
JANAINA MARQUES DE ALMEIDA; VIVIAN ROTUNO MOURE; GABRIELA TONETTO; DANIEL SÁNCHEZ; DAVID ALEXANDER MITCHELL; MARÍA LUJÁN FERREIRA; NADIA KRIEGER
Lugar:
Florianópolis
Reunión:
Seminario; ENZITEC2018; 2018
Institución organizadora:
http://www.enzitecfloripa2018.com.br/site/anaiscomplementares2?AREA=1#I
Resumen:
LipC12 is a recombinant lipase from metagenomic library that shows activity and stability as high as many commercial lipases. LipC12 is expressed with a His-tag to facilitate its purification, but the presence of the His-tag can affect the immobilization procedure and the performance of the enzyme. In order to assess the influence of His-tag on activity, stability and immobilization of LipC12, we purified LipC12 with a His-tag at N-terminus (HisLipC12) and then removed the His-tag using tobacco etch virus (TEV) protease.