Immobilization of the LipC12 recombinant lipase, obtained by metagenomic bioprospection and its evaluation in reactions of interest in oleochemistry

KARINA BECKER LOBERMAYER; AMANDA DE SOUZA MACHADO; JANAINA MARQUES DE ALMEIDA; ROBSON CARLOS ALNOCH; GABRIELA TONETTO; DANIEL SÁNCHEZ; DAVID ALEXANDER MITCHELL; MARÍA LUJÁN FERREIRA; NADIA KRIEGER

Florianópolis

Seminario; ENZITEC2018; 2018

UFSC - Universidade Federal de Santa Catarina - e a UFFS - Universidade Federal da Fronteira Sul

In previous work, we have reported the successful expression and characterization of a lipase (LipC12) from a metagenomic library derived from a fat-contaminated soil. LipC12 is a true lipase that shows high specific activities, 1722 U mg-1against olive oil. In the present work, we immobilized LipC12 in different supports and evaluated its potential for applications in reactions of interest in the oleochemistry industry.