About the role of typical spacer/crosslinker on the design of efficient magnetic biocatalysts based on nanosized magnetite

PAULA NICOLÁS; VERONICA LASSALLE; MARÍA LUJÁN FERREIRA

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 122 p. 296 - 304

1381-1177

The immobilization of Candida antarctica lipase B (CALB) was carried out using glutaraldehyde (GLUT)and/or 3-aminopropyl-triethoxisilane (APTS). The aim of this work was to elucidate the role of these crosslinkers/functionalizers on the efficiency of the prepared nanosized catalysts in solvent-free oleic acid esterification.A series of biocatalysts were prepared in presence or absence of GLUT and APTS. The impact of the amount of initial CALB was also explored. An experimental design was utilized to study the variables that maximize biocatalyst activity.A strong dependence of enzymatic activity with the nominal amount of GLUT as well as the finalprotein/CALB loading was found. Nominal quantity of APTS did not affect catalysts activity when used in combination with GLUT. Additional studies demonstrated that stability during storage was mainly dependent on the enzyme loading. The optimum biocatalyst was reused six cycles without mass loss. Biocatalysts performance decreased with reuse. Mechanisms justifying these results were proposed.The role of GLUT and APTS on stability during storage and on differences between initial enzymaticactivity and the performance in the reaction after two months was discussed. The problem of mixed interaction of CALB (covalent bonding plus simple adsorption) was carefully addressed to explain leaching of the lipase. Leaching and stability on storage should be included in the analysis of modifiers impact when supports modifiers are used. The fresh and stored biocatalyst enzymatic activity has to be addressed looking at the practical aspects of implementation in technological settings.