Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide

SILVINA PIRILLO; ELSA HAYDÉE RUEDA; MARÍA LUJÁN FERREIRA

CHEMICAL ENGINEERING JOURNAL

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2012 vol. 204- p. 65 - 71

1385-8947

The goal of this manuscript was to study the immobilization of horseradish peroxidase (HRP) and hematin on different supports at selected conditions to be used in the removal of two synthetic dyes: Alizarin Red (AR) and Eriochrome Blue Black R (EBBR). Reuse in the target reactions was tested for the best biocatalysts. Three supports were selected: magnetite-activated carbon AFe3O4/CA, activated carbon ACA and chitosan ACSA. Several reasons led to discard the use of Fe3O4/C and C as supports for hematin and HRP. CS?glutaraldehyde (GA)HRP not only has low ability to remove EBBR, but also the enzymatic activity of HRP could not be separated from the dye adsorption on CS. However, some adsorptive functions of CSGA were lost when HRP was supported on it. In the case of CSGAhematin, the most important factors for EBBR removal are dye concentration and mass of catalyst, whereas the temperature has almost no effect on the conversion of dye, considering in all cases the H2O2 concentration (0.021 M) constant. There was not a marked decrease in the removal of AR even after the ninth reuse in the case of CSGAhematin. For EBBR removal, with CSGAHRP, in the second reuse the catalyst was completely deactivated, whereas with CSGAhematin this was found only in the fourth reuse.