INVESTIGADORES
FERREIRA Maria Lujan
artículos
Título:
Analysis Of Solvent-Free Ethyl Oleate Enzymatic Synthesis At Equilibrium Conditions
Autor/es:
V. BUCALÁ; M. L. FORESTI; G. TRUBIANO; M. L. FERREIRA; M. BRIOZZO; S. BOTTINI
Revista:
ENZYME AND MICROBIAL TECHNOLOGY
Referencias:
Año: 2006 vol. 38 p. 914 - 920
ISSN:
0141-0229
Resumen:
Este trabajo fue aceptado el 10 de Agosto del 2005. Publicado en el volumen 38 (2006). This work reports experimental equilibrium data for the esterification of pure oleic acid and a fatty acid mixture with ethanol, an using an immobilized Candida antarctica B lipase as catalyst. Reactions are performed in a solvent-free , containing a mixture of substrates and different amounts of distilled water. According to the initial amount of water and the extent of the reaction, one of two liquid phases are present. Therefore, when the equilibrium is achieved, the liquid-liquid and chemical  reaction equilibria have to be simultaneously satisfied. Several reports dealing with enzymatic reactions performed in two-phase systems have foundthat the value of the reaction equilibrium constant calculated from overall experimental concentrations varies not only with temperature but also with substrate ratio and water content. Although this approach is a valuable way to explore equilibrium shifts in biphasic systems, it is limited to ideal systems with constant partition coeficients. The aim of this work is to consider the biphasic nature of the reacive misture through a computational procedure simultaneously takes into account liquid-liquid and reaction equilibria . This approach enables  the determinatiion of a classical temperature-dependent thermodynamic equilbrium constant, which accurately fits experimental equilbrium conversions over a wide range of operating conditions.