INVESTIGADORES
FERREIRA Maria Lujan
artículos
Título:
Lipase-catalyzed acidolysis of tripalmitin with capric acid in organic solvent
Autor/es:
MARÍA LAURA FORESTI; MARÍA LUJÁN FERREIRA
Revista:
ENZYME AND MICROBIAL TECHNOLOGY
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Año: 2010 vol. 46 p. 419 - 429
ISSN:
0141-0229
Resumen:
The acidolysis of tripalmitin with capric acid catalyzed by an immobilized form of a 1,3-positionally selective lipase (Rhizomucor miehei) showed to be effective for the synthesis of structured lipids of the  MLL andMLMtype. The effects that reaction parameters such as substrate molar ratio (N), biocatalyst load  (E), and reaction temperature (T) have on selected responses variables (i.e. total conversion of tripalmitin, selectivity and yield of the desired structured lipid, hydrolysis yield, and acyl migration importance), were evaluated by use of an experimental factorial design of three factors and three levels with two central points and with a confidence level of 95%. The range of each parameter was selected as follows: N= 3–9,E = 5–15 wt%, T = 50–70C. The statistical analysis of results was addressed by use of both simple linear models and more complicated quadratic models using specific commercial software. The results obtained showed that a proper selection of reaction conditions is needed in order to maximize not only the yieldof the desired structured lipid, but also to minimize the generation of hydrolysis and acyl migration by-products.