Polyamine catabolism and H2O2 production in plant-pathogen interactions

RODRIGUEZ M; JASSO-ROBLES FI; GONZALEZ ME; PIECKENSTAIN FL; RAMÍREZ-GARCÍA JM; GUERRERO-GONZÁLEZ ML; JIMÉNEZ-BREMONT JF

Congreso; XVII Plant Biochemistry and Molecular Biology Congress & X Mexico-USA Symposium; 2017

Polyamines(PAs) are low molecular weight aliphatic polycations, with important roles inplant growth, development and stress responses. Polyamine oxidases(PAO) are FADdependentenzymes that catabolize a variety of higher PAs such as spermidine, spermineand thermospermine. Hydrogen peroxide(H2O2) is an important product of PAcatabolism, thereby linking PA degradation with cell signaling, defense responses andprogrammed cell death. Herein, the importance of PA catabolism regulation and theproduction of H2O2 in biotrophic(maize-Ustilago maydis) andhemibiotrophic(Arabidopsis-Pseudomonas) interactions are presented. In both cases, adifferential expression of members of the maize and Arabidopsis PAO gene families werenoticed. In maize, our data suggest that H2O2 produced through PA catabolism byZmPAO1(encoding en extracellular enzyme) plays an important role in tumordevelopment during the maize-U. maydis interaction. In the case of Arabidopsis,AtPAO1 and AtPAO2 genes(encoding citosolic and peroxisomal enzymes, respectively)are important in defense responses against Pseudomonas. Alteration in PAcatabolism(using T-DNA insertional lines) affects ROS production and the activities ofRBOH, SOD and catalase enzymes. Our data indicate that PA catabolism is modulateddifferentially in monocot and dicot in plant-pathogen interactions.