INVESTIGADORES
PIECKENSTAIN Fernando Luis
congresos y reuniones científicas
Título:
Spermine accumulation and oxidation increase A. thaliana tolerance to P. viridiflava
Autor/es:
GONZALEZ ME; C BORTOLOTTI, MA BLÁZQUEZ, P CARRASCO, A TIBURCIO, FL PIECKENSTAIN, OA RUIZ, OA
Lugar:
San Miguel de Tucumán, Tucumán, Argentina
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Institución organizadora:
Sociedad Argentina de Investigaciones Bioquímicas
Resumen:
Apoplastic polyamine oxidation was previously found by our group to play a role in the defense of tobacco plants against necrotrophic and biotrophic pathogens. In addition, A. thaliana plants that accumulate the tetraamine spermine by overexpression of a spermine synthase gene (35S::SPM) are less susceptible to P. viridiflava than spermine synthase null mutants (spm1-1) and wild type plants (WT). The aim of this work was to determine whether spermine-mediated tolerance of A. thaliana to P. viridiflava depends on the oxidation of this tetraamine and which polyamineoxidase (PAO) isoform is involved in this response.Feeding spm1-1 and WT A. thaliana plants with spermine led to increased tolerance to P. viridiflava, reaching a similar level to that of 35S::SPM plants. On the other hand, supplementation with the PAO inhibitor SL11061 led to increased susceptibility to P. viridiflava in the three Arabidopsis lines. Susceptibility of A. thaliana pao4-1 and pao5-1 null mutants was similar to that of WT plants, while pao1-1 null mutant exhibited increased susceptibility. The results of this work suggest that spermine-mediated tolerance of A. thaliana to P. viridiflava is due to oxidation of this tetramine by PAOs and the PAO1 isoform seems to play a specific role in this defense response. The relevance of two other PAO isoforms not evaluated in the present work remains to be established. Apoplastic polyamine oxidation was previously found by our group to play a role in the defense of tobacco plants against necrotrophic and biotrophic pathogens. In addition, A. thaliana plants that accumulate the tetraamine spermine by overexpression of a spermine synthase gene (35S::SPM) are less susceptible to P. viridiflava than spermine synthase null mutants (spm1-1) and wild type plants (WT). The aim of this work was to determine whether spermine-mediated tolerance of A. thaliana to P. viridiflava depends on the oxidation of this tetraamine and which polyamineoxidase (PAO) isoform is involved in this response.Feeding spm1-1 and WT A. thaliana plants with spermine led to increased tolerance to P. viridiflava, reaching a similar level to that of 35S::SPM plants. On the other hand, supplementation with the PAO inhibitor SL11061 led to increased susceptibility to P. viridiflava in the three Arabidopsis lines. Susceptibility of A. thaliana pao4-1 and pao5-1 null mutants was similar to that of WT plants, while pao1-1 null mutant exhibited increased susceptibility. The results of this work suggest that spermine-mediated tolerance of A. thaliana to P. viridiflava is due to oxidation of this tetramine by PAOs and the PAO1 isoform seems to play a specific role in this defense response. The relevance of two other PAO isoforms not evaluated in the present work remains to be established. Apoplastic polyamine oxidation was previously found by our group to play a role in the defense of tobacco plants against necrotrophic and biotrophic pathogens. In addition, A. thaliana plants that accumulate the tetraamine spermine by overexpression of a spermine synthase gene (35S::SPM) are less susceptible to P. viridiflava than spermine synthase null mutants (spm1-1) and wild type plants (WT). The aim of this work was to determine whether spermine-mediated tolerance of A. thaliana to P. viridiflava depends on the oxidation of this tetraamine and which polyamineoxidase (PAO) isoform is involved in this response.Feeding spm1-1 and WT A. thaliana plants with spermine led to increased tolerance to P. viridiflava, reaching a similar level to that of 35S::SPM plants. On the other hand, supplementation with the PAO inhibitor SL11061 led to increased susceptibility to P. viridiflava in the three Arabidopsis lines. Susceptibility of A. thaliana pao4-1 and pao5-1 null mutants was similar to that of WT plants, while pao1-1 null mutant exhibited increased susceptibility. The results of this work suggest that spermine-mediated tolerance of A. thaliana to P. viridiflava is due to oxidation of this tetramine by PAOs and the PAO1 isoform seems to play a specific role in this defense response. The relevance of two other PAO isoforms not evaluated in the present work remains to be established.