INVESTIGADORES
PIECKENSTAIN Fernando Luis
congresos y reuniones científicas
Título:
Functional redundancy of Arabidopsis thaliana ADC isoforms in response to Botrytis cinerea infection
Autor/es:
ROSSI FR; MARINA M; RUIZ OA; PIECKENSTAIN FL
Reunión:
Congreso; XLVIII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2012
Resumen:
Arginine decarboxylase (ADC) catalyzes the first step of polyamine (PA) biosynthesis. The model plant Arabidopsis thaliana harbors two ADC isoforms (AtADC1 and AtADC2), which are differentially regulated by different stresses, but no information about their regulation under biotic stress is available. This study aimed to determine the role AtADCs in defense against infection by Botrytis cinerea. The effect of infection on the expression of each isoform, PA levels and lesion size were evaluated in null adc1 and adc2 mutants, as compared to wild-type (WT, Col-0) plants. Infection increased the expression of both isoforms in WT plants, as compared to non-inoculated controls. AtADC2 expression was increased in infected adc1 mutants, as compared to non-inoculated adc1 plants. Reciprocally, infection enhanced AtADC1 expression in adc2 mutants, as compared to non-inoculated adc2 plants. WT plants and both adc mutants exhibited increased Put levels in response to infection, as well as similar levels of tissue damage. Results demonstrate that B. cinerea infection induces AtADC expression, but none of the isoforms seems to be defense-specific, since each one was induced by infection when the other was mutated. This functional redundancy, as well as the previously reported lethal phenotype of double (adc1-adc2) mutants render difficult to assess the contribution of ADC to resistance.