EFFECTS OF EXTRACTS OF LARREA DIVARICATA AGAINST THE STORED PRODUCT PESTS TRIBOLIUM CASTANEUM (COLEOPTERA: TENEBRIONIDAE).

RAMIREZ, ROSANA; FUCHS, LUMILA; OYHENART, JORGE

virtual

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

SAIC-SAI-SAFIS

Several shrubs belonging to genus Larrea (Zygophyllaceae) occur in semi-arid region from Argentina and are used in traditionalmedicine to treat fungal and bacterial infections. The most commonspecies is Larrea divaricata, known as female jarilla. The ethanolicextract of L. divaricata leaves has reported as antifungal when test-ed against S. cervisiae, C. albicans y A. niger. Nordihydroguaiareticacid (NDGA), found in the resin of leaves and stems, has inhibitoryeffects on the ecdysis of Rhodnius prolixus; and extends the lifecycle of Aedes Aegypti. Members of the Zygophyllaceae family havedemonstrated larvicidal activity against Cx. Pipiens, antifeedingand repellent activity against Sitophilus oryzae. However, no stud-ies were found on the bioactivity of L. divaricta against the cerealpest Tribolium castaneum (Tenebrionidae). The storage pest T. cas-taneum is a major pest of both raw and processed commodities.This study was designed to evaluate the bioactivity of L. divaricata,against the T castaneum. Samples were collected, in the provinceof La Pampa. The phytochemical extraction was performed usingorganic solvent extraction as well as aqueous extraction, accordingto conventional techniques. T. castaneum, two stages, larvae andadults were used (10 insects per well), four doses (2.5 to 50 mg)were evaluated, in triplicate. The solid resin was tested on the onehand, and the methanol extract on the other. The number of dead in-sects was recorded every 24 hours for 7 days post treatment. Com-parisons among groups of data were done using one-way ANOVA,an associated probability of P