Cultivation of Tritrichomonas foetus in bovine cervical fluid

PARADISO LANGHOFF, FRANCO; MORERO, MARIANA; RAMIREZ, ROSANA; OYHENART, JORGE

Mar del Plata

Congreso; Reunion anual SAIC-SAFE-SAB-SAP 2019; 2019; 2019

Tritrichomonas foetus is a flagellated protozoan bovine parasite. Although it can colonize other species there are no known intermediate hosts, forms of free life or resistance. T. foetus is the causative agent of sexually transmitted disease known as bovine trichomoniasis (TB). TB is common in countries where extensive bovine breeding and natural service are practiced, causing losses in calf production. In the bull the presence of T. foetus is asymptomatic while in the female it is recognized for a prolonged period between services, early abortions, vaginitis, among other aspects. In this study, the objectives were in principle the synchronization and subsequent extraction of vaginal cervical fluid (FCV) belonging to the different stages of the bovine estrous cycle; subsequently the culture of T. foetus cells in the aforementioned flow samples with the purpose of finding differences that allow us to identify an optimal culture moment, if this exists. After the analysis of the data, it was possible to observe that the FCV allows cell growth, counted at 48 hours, and does so differentially according to the stage of the estrous cycle to which the sample corresponds. Based on these results, it is possible to conclude that the proposed model is appropriate for the initially proposed objectives