"Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/DAPI banding, and inferences about their karyotype differentiation

CELESTE, SILVESTRI MARÍA; ORTIZ, ALEJANDRA MARCELA; ROBLEDO, GERMÁN; LAVIA, GRACIELA INÉS

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS.

ACAD BRASILEIRA DE CIENCIAS

Lugar: Rio de Janeiro; Año: 2020

0001-3765

"The species of the genus Arachis (Leguminosae) are ordered into nine sections.The assignment of genome types in this genus has been based on cross-compatibilityanalysis and molecular cytogenetic studies. The latter has also allowed karyotypicallyestablishing well-defined genomes and reassigning the genome of several species.However, most of these studies have been focused mainly on the sections Arachisand Rhizomatosae. To increase the knowledge about the chromosome diversity of thewhole genus, here we performed a detailed karyotype characterization of representativespecies of most of the sections and genomes of Arachis. This characterization includedchromosome morphology, CMA/DAPI chromosome banding, and chromosome markerlocalization (rDNAloci and one satDNA sequence) by fluorescent in situ hybridization(FISH). Based on the data obtained and other previously published data, we establishedthe karyotype similarities by cluster analysis and defined eleven karyotype groups.The grouping was partly coincident with the traditional genome assignment, exceptfor some groups and some individual species. Karyotype similarities among somegenomes were also found. The main characteristics of each karyotype group of Arachiswere summarized. Together, our results provide information that may be beneficial forfuture cytogenetic and evolutionary studies, and also contribute to the identification ofinterspecific hybrids