INVESTIGADORES
VIZIOLI nora Matilde
congresos y reuniones científicas
Título:
Changes in the methylation profile of DNA extracted from brain tissues of rats subjected to exposure of trace elements
Autor/es:
RATTI, S; GAGLIO, E.; GRELA D; ALVAREZ, EO; VIZIOLI, NM
Lugar:
Buenos Aires
Reunión:
Simposio; 18th Symposium on Biomedical, Biopharmaceutical and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2012
Resumen:
Recently, children from a certain area in La Rioja, province of Argentina, characterized by the abundance of mineral deposits, were found to have altered phenotypic expression attributed to the HSR (Hand Skill, Relative; OMIM 139900) gene (1). This gene has been found to be associated to handedness, brain asymmetry, reading-writing abilities and susceptibility to schizophrenia. A hypothesis was raised considering the epigenetic regulation of the HSR gene and its susceptibility to environmental influences; trace elements present in this region could be exogenous factors involved in the altered expression attributed to this gene. Thus, the objective of the present work was to test in a cognitive lateralization and associate behavioural responses rat model, the possible biological effect of ZnTe as a representative trace element on some spontaneous and natural behavioural responses simile to HSR expression of humans. ZnTe treatment (0.03 µg/L - 3 µg/L) was applied in drinking water to pregnant mother animals along all gestation, delivery, weaning and preadolescent periods. Results showed that ZnTe treatment produced two opposing effects. On one hand, treated young rats displayed excitatory motor and selective motivated exploration responses in a behavioural automatic activity device, and on the other hand, impairments in motivated and lateralized behavioural display in a lateralized exploratory labyrinth. In other different test measuring defensive behaviour, natural defensive responses were attenuated in ZnTe treated rats. Determination of the methylation pattern of DNA in prefrontal cortex and hippocampus was performed by capillary electrophoresis and showed that ZnTe treatment modified the ratio of non-methylated- to methylated cytosine, suggesting an epigenetic change on the same line to that observed in children in the previous study of this laboratory. In conclusion, the behavioural rat model used in the present study confirms some of the previous evidence found in humans. References 1. Ratti SG, Vizioli NM, Álvarez EO. Am. J. Neuroprotec. Neuroregen. 2010, 2 (1), 65-70