INVESTIGADORES
VIZIOLI nora Matilde
congresos y reuniones científicas
Título:
Determination of diffusion coefficient of plasmatic proteins by capillary electropho
Autor/es:
VIZIOLI NM; YONE A; HIDALGO R; GRASSELLI M
Lugar:
Guarujá, Brasil
Reunión:
Simposio; 11th Symposium on Biomedical, Biopharmaceutical and Industrial Applications of Capillary Electrophoresis and Microchip Technology, LACE 2005; 2005
Resumen:
Bioartificial extra-corporeal systems make use of cell reactors that are separated from surrounding media solutions by porous polymeric membranes in hollow fiber format. Such porous structures control the passage of substances, depending on their molecular size, by different mass transfer processes like convection, diffusion and osmosis. The goal in the design of these systems is to obtain membranes that allow the differential diffusion of plasmatic proteins with a negligible immunoglobulin passage through. The experimental approaches provide very little amount of solutions in which the level of different substances must be determined following characterization purposes. On this matter, capillary electrophoresis (CE) represents a very useful tool since different analytes can be separated and simultaneously quantified in minute samples. New membranes based on hydrogels confined into macroporous systems were developed by in situ radio-initiated polymerization and crosslinking of: a) hydroxyethyl methacrylate and diethylene glycol dimethacrylate, and b) acrylamide and ethylene bisacrylamide. Concentration of HSA and IgG was determined at different times in the receiving reservoir by CE. A sodium phosphate buffer (pH 8.3) containing lithium chloride was used as background electrolyte and the separations was performed at 25°C with UV detection (214 nm). Diffusion coefficients were calculated from plots of protein concentration versus time and served as a critical parameter for membrane-supported hydrogel characterization.