INVESTIGADORES
BELLORA nicolas
artículos
Título:
Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB
Autor/es:
MIGUEL B COELHO; JAN ATTIG; NICOLÁS BELLORA; JULIAN KÖNIG; MARTINA HALLEGGER; MELIS KAYIKCI; EDUARDO EYRAS; JERNEJ ULE; CHRISTOPHER WJ SMITH
Revista:
EMBO JOURNAL
Editorial:
NATURE PUBLISHING GROUP
Referencias:
Lugar: Londres; Año: 2015
ISSN:
0261-4189
Resumen:
Matrin3 is an RNA- and DNA-binding nuclear matrix protein foundto be associated with neural and muscular degenerative diseases.A number of possible functions of Matrin3 have been suggested,but no widespread role in RNA metabolism has yet been clearlydemonstrated. We identified Matrin3 by its interaction with thesecond RRM domain of the splicing regulator PTB. Using a combinationof RNAi knockdown, transcriptome profiling and iCLIP, wefind that Matrin3 is a regulator of hundreds of alternative splicingevents, principally acting as a splicing repressor with only a smallproportion of targeted events being co-regulated by PTB. Incontrast to other splicing regulators, Matrin3 binds to an extendedregion within repressed exons and flanking introns with no sharplydefined peaks. The identification of this clear molecular function ofMatrin3 should help to clarify the molecular pathology of ALS andother diseases caused by mutations of Matrin3.