INVESTIGADORES
OLIVERA nelda Lila
artículos
Título:
Novel Insights about Class 2 Integrons from Experimental and Genomic Epidemiology
Autor/es:
MARIA SOLEDAD RAMÍREZ; PIÑEIRO, S.; KAUFMAN, S.; KOVENSKY, J.; VAY, C.; TOKUMOTO, M.; FERNÁNDEZ, A.; FAMIGLIETTI, A.; COUTO, E.; LOPARDO, H.; SMAYEVSKY, J.; ALMUZARA, M.; PROCOPIO, A.; VÁZQUEZ, M.; NELDA LILA OLIVERA; DE GROSSI, J.; FERREIRO, M.; FLAIBANI, M.; FAVRE, R.; CASSINI, M.H.; IGLESIAS, M.; DI MARTINO, A.; CATALANO, M.; VARGAS, L.J.; DE PAULIS, A.; PREDARI, S.; DANIELA CENTRON
Revista:
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Año: 2010 vol. 54 p. 699 - 699
ISSN:
0066-4804
Resumen:
In order to contribute to the knowledge of the architecture and epidemiology of class 2 integrons, we performed a class 2 integron molecular survey in which we analyzed 726 isolates in two bacterial populations from environmental and nonepidemiologically related clinical samples, respectively, collected from 1982 to 2007. We recovered the intI2 gene from 130 of 726 isolates, most of which were clinical isolates, and only 1 (a psychrophilic Pseudomonas sp.) was from a water sample. Unlike the widespread distribution of class 1 integrons within Gram-negative bacilli, only Acinetobacter baumannii and Enterobacter cloacae harbored class 2 integrons at a high frequency in our collection. Class 2 integrons with six novel cassette arrays were documented. Characterization of the transposition module of Tn7, the genetic platform in which class 2 integrons have always been reported, showed tns modules with a mosaic genetic structure. A bioinformatic analysis performed with the tns genes present in sequence databases, the finding of intI2 not associated with tns genes, and the genetic examination of novel tns-like genes found in three isolates indicated the possibility of the independent evolution of the two components, the class 2 integrons and the Tn7 transposons, in association with horizontal gene transfer.