Cyclolepis genistoides aqueous extract as source of neuroprotective agents

ALZA, NATALIA P.; CAVALLARO, VALERIA; ORIANA BENZI JUNCOS; ANA PAULA MURRAY; SALVADOR, GABRIELA A.

Congreso; 7th International Electronic Conference on Medicinal Chemistry; 2021

MDPI

Sinceoxidative stress ( is a main component in neurodegenerative diseases, targeting thiscausative agent constitutes an important approach in drug discovery In previous studies, we haveselected the aqueous extract of Cyclolepis genistoides from a set of medicinal plants based on itsability to diminish metal induced OS cellular markers The aim of this work was to characterize themechanism by which C genistoides extract protected cells against OS For this purpose, humanneuroblastoma cells (IMR 32 were exposed to C genistoides extract under conditions favoring OS(ferric ammonium citrate FAC exposure) C genistoides extract 20 μg / diminished thegeneration of superoxide anion in the presence of FAC Moreover, the extract increased catalaseactivity when cells were exposed to FAC Additionally, C genistoides extract triggered the nucleartranslocation of Nrf 2 a cytoprotective transcription factor involved in antioxidant enzymeexpression T he determination of flavonoids in C genistoides extract revealed 16 1 0 1 mgquercetin equivalents/g To identify the bioactive components, 9 fractions (A I) were obtainedafter a bio guided fractionation The evaluation of the main fractions F, G and H 20 μg /showed that fractions F and H exposure reduced reactive oxygen species ( productioninduced by FAC however, fraction G treatment increased ROS levels Until now, a sesquiterpenelactone was identified in fraction F Our findings suggest that C genistoides extract exerts theprotective effect via the activation of cellular antioxidant defenses Further studies are necessaryto identify which compounds are responsible for the neuroprotective effect through Nrf 2modulation