MURRAY Ana paula
congresos y reuniones científicas
Essential oil from fruits and leaves of Schinus areira L. with acetylcholinesterase inhibitory activity
Graz, Austria
Congreso; 55th International Congress and Annual Meeting of the Society for Medicinal Plant Research; 2007
Institución organizadora:
Society for Medicinal Plant Research
Currently available drugs for the symptomatic treatment of Alzheimer´s disease (AD) are based on the inhibition of the enzyme acetylcholinesterase (AChE). Recently, essential oils as well as terpenoids have been shown to inhibit AChE in in vitro assays [1], [2]. In the present communication we are reporting the evaluation of the essential oils from fruits and leaves of Schinus areira L (Anacardiaceae), extensively used in folk medicine in South America [3], for their activity towards AChE. The enzymatic activity was evaluated using an adaptation of a previously described method [4]. The composition of the essential oils was determined by GC and GC-MS [5]. The essential oil from fruits showed a 29.00±4.84% AChE inhibition while the essential oil from leaves showed a 13.67±0.67% AChE inhibition under the same conditions (0.1µL/mL). Chromatographic fractionation of the essential oils guided by the bioassay led to the isolation of the active fractions. GC-MS analysis of those fractions revealed the presence of sesquiterpenes like â-eudesmol and elemol, which have been reported as compounds that may have potential therapeutic use against amnesia-inducing diseases like AD [6], [7]. Acknowledgements: CONICET, ANPCYT, SECYT-UNS. References: [1] Perry, N.S.L et al. (2003) Pharmacol Biochem Behav 75: 651–659. [2] Miyazawa M., Yamafuji C. (2005) J. Agric. Food Chem. 53: 1765–1768. [3] Gupta M.P. (1995) 270 Plantas Medicinales Iberoamericanas. Ed. CYTED, D.C. Colombia. [4] Rhee I. K. et al. (2001) J. Chromatogr. A 915: 217–223. [5] Murray A. P. et al. (2005) Z. Naturforsch. 60C: 25–29. [6] Obara Y. et al. (2002) J. Pharmacol. Exp. Ther. 301: 803–811 [7] Kim K et al. (2006) Biosci Biotechnol Biochem. 70: 1821–1826.