INVESTIGADORES
MURRAY Ana paula
congresos y reuniones científicas
Título:
Antibacterial and antioxidant activities of essential oils isolated from Schinus areira L. (Anacardiaceae)
Autor/es:
RODRIGUEZ, SILVANA; SUEIRO, R.A.; ANA PAULA MURRAY; LEIRO J.M.
Lugar:
Rio de Janeiro
Reunión:
Simposio; 3rd International Symposium on Challenges and New Technologies in Drug Discovery & Pharmaceutical Production; 2015
Institución organizadora:
Fundação Oswaldo Cruz. Ministério da Saude
Resumen:
The present study was carried out to evaluate the possible interactions on antibacterial and antioxidant efficiency of essential oils of Schinus areira L. (Anacardiaceae) species. The essential oils of fruits and leaf have been studied for their antibacterial activity against twelve important bacteria: Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, Salmonella enteriditis, Pseudomonas aeruginosa, Shigella sonnei, Proteus vulgaris, Enterococcus faecium, Enterococcus faecalis, using microbroth dilution method. Antioxidant combination effect was assessed by DPPH free radical scavenging method and crocin bleaching assay. Bioactivity?guided fractionation of active essential oils for isolation of bioactive compounds was done using TLC-bioautography assay and RP-HPLC. Chemical characterization of bioactive compounds was performed using CG-MS, HPLC-MS and NMR analyses. Results showed that the bioactive isolated compounds, myrcene, β-caryophyllene, globulol, α-phellandrene and β-pinene, exhibit both antibacterial and antioxidant activities. These compounds showed antibacterial activity with minimal inhibitory concentration between 0,37 and 5 µg/mL. Results of antioxidant activity indicate that the terpenoids possess excellent DPPH free radical scavenging activity with IC50 values from 5,27 to 21,20 μg/ml, while for crocin bleaching assay Krel ranged from 0,80 to 1,84 μg/ml. The results provide evidence that the oils from Schinus areira might be used as a potential source of safe and effective natural antimicrobial and antioxidant agents in pharmaceutical and food industries.