Antimutagenic and promutagenic activity of Limonium brasiliense (Boiss) Kuntze extract

RODRIGUEZ, SILVANA; SUEIRO, R.A.; ANA PAULA MURRAY; LEIRO J.M.

Córdoba

Congreso; 3rd International Meeting on Pharmaceutical Sciences; 2014

Colegio de Farmacéuticos de Córdoba

Limonium brasiliense Kuntze (Plumbaginaceae) is a medicinal plant, known as ?Guaycuru? from southern Argentina. Infusion from the roots is popularly used in the treatment of hemorrhage, menstrual disorders, genito-urinary infections and cardiac diseases. The increasing research on the isolated active components in this plant reveals a large number of components in the roots, which have been the subject of many studies that show biological activity. To these active compounds are attributed antioxidant, and antiinflammatory properties. We have also found antimutagenic properties in vitro and ex vivo, being the main objective in the present study. The mutagenicity of the extract and the isolated components of L. brasiliense were evaluated using the Ames assay with strains Salmonella typhimurium TA98, TA100, TA102, TA1535 and TA1537 in the absence and presence of exogenous metabolic activation (S9 fraction from rat liver), at concentrations between 50 - 0.05 mg/mL. In the tests without metabolic activation, the mutagens utilized were sodium azide (NaN3), 2,4,7-trinitro-9-fluorenone (TNF), 4-nitro-0-phenylenediamine (NPD), 4-nitroquinoline N-oxide (4NQO), methylmethanesulfonate (MMS) and mitomycin C, while in the tests with metabolic activation, the mutagen 2 amine acridine (2AA) was used. The entire assay was performed in triplicate. The aqueous extract and the compounds isolated from the roots of L. brasiliense (myricitrin and epigallocatechin gallate dimer (EGCG)), acted as strong antimutagens against the mutagens previously mentioned. In the absence of metabolic activation their inhibition resulted in a range from 61.76% to 86.02 %. While the inhibition of mutagenic effect with metabolic activation, resulted in a range from 68.93%-95.01 %, calculated in the same concentration. These results demonstrate that EGCG dimer, myricitrin and the aqueous extract from the roots of L. brasiliense are not mutagenic. They are able to reduce or inhibit the lesions in the DNA or mutations that induce cancer cells and diseases caused by genotoxic agents.