INVESTIGADORES
MOLDES carlos alberto
artículos
Título:
Physiological effects of Glyphosate over amino acid profile in conventional and transgenic soybean (Glycine max).
Autor/es:
CARLOS ALBERTO MOLDES; JOSÉ MANUEL CAMIÑA; LEONARDO OLIVEIRA MEDICI; SIU MUI TSAI; RICARDO ANTUNES AZEVEDO
Revista:
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: New York; Año: 2012 p. 134 - 141
ISSN:
0048-3575
Resumen:
This paper compares the responses of conventional and transgenic soybean to glyphosate application in terms of the contents of 17 detectable soluble amino acids in leaves, analyzed by HPLC and fluorescence detection. Glutamate, histidine, asparagine, arginine + alanine, glycine + threonine and isoleucine increased in conventional soybean leaves when compared to transgenic soybean leaves, whereas for other amino acids, no significant differences were recorded. Univariate analysis allowed us to make an approximate differentiation between conventional and transgenic lines, observing the changes of some variables by glyphosate application. In addition, by means of the multivariate analysis, using principal components analysis (PCA), cluster analysis (CA) and linear discriminant analysis (LDA) it was possible to identify and discriminate different groups based on the soybean genetic origin.