INVESTIGADORES
MICIELI Maria Victoria
artículos
Título:
Protocol for experimental infections with viruses in mosquitoes using a glass artificial feeder
Autor/es:
BONICA, MELISA B.; MARTI, GERARDO A.; GOENAGA, SILVINA; BALSALOBRE, AGUSTÍN; MICIELI, MARÍA V.
Revista:
STAR Protocols
Editorial:
CellPress
Referencias:
Lugar: Cambridge; Año: 2025 vol. 6
ISSN:
2666-1667
Resumen:
Here, we present a protocol for infecting Aedes aegypti mosquitoes with Zika virus(ZIKV) (Flaviviridae) using a glass artificial feeder that mimics natural feedingconditions. We describe steps for preparing adult mosquitoes, assembling thefeeder, mixing the infectious blood meal, and conducting infection experiments.The feeder incorporates a bovine gut membrane and a thermostatic circulator tomaintain the blood meal at 37◦C, ensuring consistent feeding and reproducibleinfection outcomes.For complete details on the use and execution of this protocol, please refer toBonica et al
