INVESTIGADORES
MERONI silvina Beatriz
congresos y reuniones científicas
Título:
Assessment of the role of L-glutamine in the regulation of Sertoli cell proliferation
Autor/es:
GALARDO MARÍA NOEL; GORGA AGOSTINA; RINDONE, GUSTAVO MARCELO; PELLIZZARI ELIANA HERMINIA; CAMBEROS MARÍA DEL CARMEN; CIGORRAGA SELVA BEATRIZ; RIERA MARÍA FERNANDA; MERONI SILVINA BEATRIZ
Lugar:
Mar del Plata
Reunión:
Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2018
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
The final number of Sertoli cells (SC) reached during the proliferative periods determines sperm production capacity in adulthood. On the other hand it is well known that nutrient availability plays a pivotal role in the decision of a cell to commit to cell proliferation. Crucial to the decision process is the mammalian target of rapamycin complex 1 (mTORC1), whose activity is controlled by, besides hormones such us FSH, the presence of essential amino acids (aa). L-glutamine (gln), a non-essential aa, plays an important role in proliferation in many cell types. However, the role of gln in the regulation of SC proliferation has not been studied yet. The aim of this work was to investigate whether SC depends on the presence of gln to proliferate.SC obtained from 8-day old rats were maintained in the absence or presence of gln (2.5mM) and stimulated with FSH 100ng/ml. BrdU incorporation, cyclin D2 (CCND2) expression by RT-qPCR and phosphorylated mTOR (P-mTOR) levels by western blot were evaluated. Results are expressed as mean±SD of three independent experiments (different letters indicate statistically significantdifferences, P