INVESTIGADORES
MERONI silvina Beatriz
congresos y reuniones científicas
Título:
EFFECT OF GLUCOSE DEPRIVATION ON SERTOLI CELL LIPID METABOLISM
Autor/es:
RINDONE GUSTAVO; GORGA AGOSTINA; REGUEIRA MARIANA; PELLIZZARI ELIANA HERMINIA; CAMBEROS MARÍA DEL CARMEN; GALARDO MARÍA NOEL; MERONI SILVINA BEATRIZ; RIERA MARÍA FERNANDA
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de BioCiencias; 2017
Institución organizadora:
Comité Organizador Reunión Conjunta de Sociedades de BioCiencias
Resumen:
Sertoli cells (SC) possess a unique metabolism based on its role as nutritional support for germ cells. SC actively metabolize glucose into lactate to provide energy to cells located in the adluminal compartment of seminiferous tubules. Additionally, it has been demonstrated that SC can oxidize fatty acids (FA) and also store them as triacylglycerides (TAG) within lipid droplets (LD). We have previously demonstrated that SC are able to survive in culture for at least 48 h in the absence of glucose suggesting that, under this adverse condition, SC lipid metabolism would become essential for survival. However, no experiments regarding this matter were performed. The aim of the study was to investigate the effect of glucose deprivation on the regulation of lipid catabolism in SC. For this purpose, SC isolated from 20-day-old rats were cultured for 48 h in media containing different concentrations of glucose: 8 mM (concentration commonly used in the culture media of SC) and 0 mM. FA oxidation (measured as 3H2O production from [9,10-3H(N)]-palmitic acid), carnitine palmitoyltransferase 1A (CPT1A) expression (RT-qPCR), TAG levels (spectrophotometric method) and LD content (Red Oil O staining) were evaluated. Results are shown as means±S.D. of triplicate incubations in one representative experiment out of three. (*P