INVESTIGADORES
BRECCIA Javier Dario
congresos y reuniones científicas
Título:
Polyvinyl alcohol and pectin cryogel two-layer patch for controlled release of enrofloxacin
Autor/es:
MARTINEZ Y,; PINUEL L; CASTRO GR,; BRECCIA JD
Reunión:
Congreso; Applied Biochemistry and Biotechnology. 5th Trivandrum Biotechnology Conference - NEW HORIZONS IN BIOTECHNOLOGY- NHBT-2011-; 2011
Institución organizadora:
Biotech Research Society
Resumen:
Cryogel patches composed of polyvinyl alcohol and pectin (PVA-P) blend were produced by repeating cycles of freeze–thawing. Since the pH in mammals’ skin is found in the range of 4.2 to 5.6, the release of enrofloxacin entrapped in the PVA-P cryogel matrix was evaluated at pH 5.5. The PVA cryogel has zero-order kinetic for enrofloxacin release and liberate 69.7% of the antibiotic in 4.5 h at 37°C. To slow down the quinolone release rate, high metoxylated pectin was added into the cryogel. This biopolymer showed a high binding capacity to the cationic form of the antibiotic. When 5.0 µg/ml of enrofloxacin were loaded, only 3.7% was released from a patch containing 1.0% (w/v) pectin after 4.5 h. Therefore, a two layer-system was designed to modulate the releasing rate of the antibiotic. In this system the top film was loaded with NaCl (1.5M) and the diffusion of the electrolyte to the bottom film containing the drug improved the release rate (24.0% in 4h). The release rate of enrofloxacin was found dependent of NaCl concentration in the upper gel layer. Consequently the new two-layer cryogel system showed attractive features for transecutaneous drug delivery.