INVESTIGADORES
BRECCIA Javier Dario
congresos y reuniones científicas
Título:
Transglycosylation of terpenes in cultures of Acremonium sp. DSM24697
Autor/es:
BRECCIA JD
Reunión:
Conferencia; SAMIGE2017; 2017
Resumen:
Uncommon glycoside hydrolases: biotransformations in food and bioprocesses Javier D. BrecciaINCITAP (CONICET- UNLPam), Departamento de Química, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa (UNLPam), Av. Uruguay 151, (6300) Santa Rosa, La Pampa, Argentina. javierbreccia@gmail.comThe most common strategy for deglycosylation of flavonoids and other secondary metabolites in plants goes through monoglycosidases that release the monosaccharides before the oxidation of the aglycone. However a less common path is the deglycosylation through an unique enzyme. These enzymes called diglycosidases, are endo-β-glucosidases that hydrolyze the heterosidic linkage of diglycoconjugates, splitting off a disaccharide and the corresponding aglycone. As an example, the fungus Acremonium sp. DSM 24697 produces at least two diglycosidases with different substrate specificity: α-rhamnosyl-β-glucosidase (ARBG) cleaves the disaccharide rutinose from 7-O-rutinosylated flavonoids, while ARBGII cleaves rutinose from the 3-O-rutinosylated flavonoid rutin (Fig 1). The existence of these enzymes was predicted in the beginnings of the XX century, and just six decades later was shown that the reaction is performed by an unique enzyme. The systematic studies began in the new century and up to now 4 diglycosidase activities were reported. These enzymes hydrolyze the disaccharides primeverose, acuminose, rutinose and vicianose. The plant diglycosidases belong to GH1 while fungal enzymes belong to GH5 subfamily 23. Recently we reported a hesperidin 6-O-α-L-rhamnosyl-β-D-glucosidase from bacteria that belong to the family GH55. The capacity of release the disaccharide in one reaction as well as the transglycosylation activity makes these biocatalysts an interesting toolbox for food and bioactive molecules biotransformations. Figure 1. Structure of flavonoids (A) rutin (quercetin 3-O-(6-O-α-L-rhamnopyranosil-β-D-glucopyranoside) (B) hesperdidin (hesperetin 7-O-(6-O-α-L-rhamnopyranosil-β-D-glucopyranoside)).This work was supported by the National University of La Pampa (UNLPam), the National Council of Scientific and Technical Research (CONICET), and The National Agency for Science and Technology Promotion (ANPCyT) of Argentina.