INVESTIGADORES
BRECCIA Javier Dario
congresos y reuniones científicas
Título:
Deglycosylation of flavonoids by Hypocreales strains
Autor/es:
BRECCIA JD
Reunión:
Conferencia; SAPROBIO2018; 2018
Resumen:
Acremonium is a heterogeneous genus among fungal diversity and its taxonomy changed significantly in the last three years. A screening of fungal diglycosidases in the genera Acremonium and Sarocladium revealed strains wearing diglycosidase activity. This less common path of deglycosylation through an unique enzyme is catalyzed by diglycosidases, endo-β-glucosidases that hydrolyze the heterosidic linkage of diglycoconjugates, splitting off a disaccharide and the corresponding aglycone. As an example, the fungus Acremonium sp. DSM 24697 produces at least two diglycosidases with different substrate specificity: α-rhamnosyl-β-glucosidase (ARBG) cleaves the disaccharide rutinose from 7-O-rutinosylated flavonoids, while ARBGII cleaves rutinose from the 3-O-rutinosylated flavonoid rutin. The systematic studies of these enzymatic systems were promoted in the last 10 years and up to now 4 diglycosidase activities were reported. These enzymes hydrolyze the disaccharides primeverose, acuminose, rutinose and vicianose. The plant diglycosidases belong to GH1 while fungal enzymes belong to GH5 subfamily 23. Recently we reported a hesperidin 6-O-α-L-rhamnosyl-β-D-glucosidase from bacteria that belong to the family GH55. The capacity of release the disaccharide in one reaction as well as the transglycosylation activity makes these biocatalysts an interesting toolbox for food and bioactive molecules biotransformations.