Dose-dependent significance of monosaccharides on intracellular α-L-rhamnosidase activity from Pseudoalteromonas sp. 005NJ

MAZZAFERRO LS; ORRILLO GA; LEDESMA P; BRECCIA JD

BIOTECHNOLOGY LETTERS

Springer

Lugar: Netherlands; Año: 2008 vol. 30 p. 2147 - 2150

0141-5492

Intracellular a-L-rhamnosidase (EC3.2.1.40) from the psychrotolerant Pseudoalteromonassp. 005NJ showed a dose-dependent inhibition for L-rhamnose (IC50= 20mM) and D-ribose (IC50 = 95mM), whereas D-glucose and L-fucose presented a lower inhibition, with IC50  values as high as 0.5 M and 0.2M, respectively. On the other hand, D-fructose enhanced enzyme activity three fold, reaching a plateau of maximum specific activity between 0.2 and 0.4M of this monosaccharide. Both effects, low inhibition and stimulation, caused by key fruit sugars (glucose and fructose),make this biocatalyst an interesting system in terms of its potential application for debittering fruit juices.