congresos y reuniones científicas
SUGAR DERIVATIZATION OF POLYALCOHOLS USING THE FUNGAL ENZYME α-RHAMNOSYL-β-GLUCOSIDASE
JAN-MATHIS MÜLLER; ALEXANDER FRIES; JAVIER D. BRECCIA; MICHAEL MÜLLER; LAURA S. MAZZAFERRO
Jornada; XII Jornadas Pampeanas de Ciencias Naturales; 2016
Consejo Profesional de Ciencias Naturales de La Pampa
The fungal diglycosidase α-rhamnosyl-β-glucosidase has proven to be capable of catalyzing transglycosylation reactions using alcohols as sugar acceptors and hesperidin as sugar donor. Glycerol is especially interesting as sugar acceptor, as it is a byproduct of the biodiesel synthesis and thus easily and in vast amounts available. In this work, we tested the polyalcohols ethylene glycol and glycerol as sugar acceptors. Under standard conditions (1.8 mM hesperidin, 40% alcohol, 0.02 U/mL enzyme, 2 h, 60 °C), the corresponding rutinosylated products were obtained (ca. 37% conversion regarding hesperidin with glycerol and ca. 52% with ethylene glycol). By optimizing the reaction conditions (temperature, incubation time, effect of shaking) a conversion higher than 90% was obtained with both acceptors. Since glycerol and ethylene glycol contain multiple alcoholic groups, the transglycosylation products were analyzed using mass spectrometry. In both cases, the enzyme was found to transglycosylate only one alcoholic group. Because of the properties of sugar glycerides, such as protection against oxidative and osmotic stress, they are considered as possible skin-moisturizers and underline the possible use of rutinosylated glycerol in the cosmetic industry.