INVESTIGADORES
SCERVINO Jose martin
congresos y reuniones científicas
Título:
THE ROLE OF THE MYCORRHIZAL FUNGUS RHIZOPHAGUS CUSTOS IN THE DISSIPATION OF PAHS UNDER ROOT-ORGAN CULTURE CONDITIONS
Autor/es:
ARANDA E; J. M. SCERVINO; M. S. GODOY; WITTICH R.; J. A. OCAMPO; GARCIA-ROMERA I
Lugar:
Lipzig
Reunión:
Congreso; The 5th Congress of European Microbiologists (FEMS 2013); 2013
Institución organizadora:
Asociación europea de microbiologos
Resumen:
Mycorrhiza arbuscular fungi are obligate biotrophes for higher plants. They participate in several physiological processes on plants, including alleviation of pollutant toxicity. The fungus Rhizophagus custos −isolated from a metal contaminated soil− confers protection to host plants in terms of resistance to extreme pH and heavy metal concentrations in soil, and in consequence, this fungus could have developed some strategies for pollutant resistance. Polycyclic aromatic hydrocarbons (PAHs) are one of the most common contaminants on soil. This work is aimed at evaluating the impact of two isomer 3-ring PAHs−anthracene (Ant) and phenanthrene (Phe)− in comparison to a heterocyclic aromatic hydrocarbon −dibenzothiophene (Dbt)− in non-mycorrhizal carrot roots as well as in mycorrhizated roots with the fungus R. custos. Its effects on mycorrization parameters under in vitro root-organ culture conditions were studied. Different amounts of Ant, Phe and Dbt were added to the monoxenic culture and the residual content after 42 days of cultivation, on the media as well as on roots, were analyzed chromatographically. The percentage of root colonization, hyphen development and mycorrhizal parameters has been discussed in terms of PAHs dissipation.Our results showed not negative effect of Ant on the root dry weight in mycorrizated root. The toxicity of these compounds negatively affects to the hyphal development and the functionality of symbiosis, showing a reduction of 20% on percentage of root colonization exceeding molarities of 120 μM. However, these monoxenic experiments demonstrated a positive contribution of the symbiotic association to PAHs dissipation in the absence of the symbiotic association to PAHs dissipation in the absence of other microorganisms.