INVESTIGADORES
SUAREZ maria laura
congresos y reuniones científicas
Título:
Genomic markers as indicators of potential drought adaptation in Nothofagus dombeyi forests of northern Patagonia
Autor/es:
FASANELLA, MARIANA; SUAREZ, MARIA LAURA; HASBUN, RODRIGO; PREMOLI, ANDREA C.
Reunión:
Conferencia; Topwood; 2019
Resumen:
Global change type-droughts have been recorded in Patagonia triggering high mortality of Nothofagus dombeyi (coihue) forests. Drought stress is likely to affect survival and fitness of trees causing genetic differences depending on the degree of drought. The mechanisms of how plants respond to drought are controlled by many genes so genome-wide approaches offer a more complete picture. Combining dendrochronology (e.g. relative resilience-RRs) with genetic association allowed us to assess tree vulnerability to past drought events at fine temporal scales, and provides avenues for future genomic studies on functional adaptation in forest trees. The aim of this study is to identify functional candidate genes for drought stress response in coihue, as well as the association of polymorphic candidate genes with dendrophenotypic traits. In particular, we are interested in identifying whether differences in individual reactions are associated with genetic variation among individuals (healthy crown-HC and partial crown-dieback-PD). We collected fresh leaves and extracted wood cores from 19 pairs of individuals (HC and PD) from 4 sites affected by the 1998 and 2014 droughts. A total of 5224 SNPs were obtained, of which 33 allowed to clearly discriminate HC from PD; 8 of these related to gene response to hydric stress. An association analysis between genetic and dendrophenotypic traits was performed, obtaining a total of 3 SNPs associated to RRs. Our results would indicate that susceptibility to drought in coihue could be determined at the genetic level. The combination of both approaches could provide a framework that can contribute to the identification and analysis of candidate genes for stress response in coihue and other non-model species.